Polish Journal of Natural Sciences

EMAMECTIN BENZOATE EXPOSURE IMPAIRED GERM CELL MATURATION IN THE TESTES OF ADULT ALBINO RATS

Assia Kamel — 2024-12-23

Certain pesticides represent a substantial risk to reproductive function, and their possible reprotoxic effects have been the focus of numerous research. Emamectin Benzoate (EMB), a biopesticide widely used in agriculture, household items, and veterinary health, can cause tissue damage in the male reproductive system due to oxidative toxicity. The purpose of this study was to investigate the effects of Emamectin Benzoate toxicity in male Wistar rats. Fourteen rats were divided into two groups of seven each. For 15 days, the EMB group received a daily dose of EMB corresponding to 20 mg/Kg by gavage. Distilled water was given to the control group. Oxidative stress, DNA fragmentation, histopathology and germ cell degeneration of testis were investigated. Emamectin administration induced oxidative stress in testicle evidenced by elevated levels of MDA, protein carbonyl and percentage of DNA fragmentation with depletion of the defense system (CAT and SOD activities). Emamectin produced several histopathological changes in the testis, these results suggest that the subchronic exposure to emamectine benzoate affects the redox state which contribute to testicular impairment.

EFFECT OF EXTENDER AND DILUTION RATIO ON THE SPERM MOTILITY, VIABILITY, AND EGGS FERTILITY OF CLARIAS BATRACHUS, LINNAEUS 1758 (PISCES: CLARIIDAE)

Luvi S Handayani — 2024-12-23

Refrigeration is a simple preservation technique for the short-term storage of fish sperm. The suitable extender and dilution ratio are crucial during this process. Therefore, this study aims to determine the type of extender and the best dilution ratio for preserving walking catfish Clarias batrachus sperm at a refrigeration temperature of 4 °C. The study employed a completely randomized design with two factors, each consisting of 6 treatments and 3 replications. The experiments were divided into 2 stages: the first stage tested several types of extenders, namely tap water, Ringer's solution, physiological solution, Alsever's solution, urea solution, and glucose base solution, all at the same dilution level of 1:20 (sperm:extender, v/v). The second stage examined various levels of sperm dilution ratios, namely 1:10, 1:20, 1:30, 1:40, 1:50, and 1:60 (sperm:extender, v/v). The samples were stored at a refrigerated temperature of 4 °C. Furthermore, motility and viability were monitored at 24-hour intervals for 144 hours, and on the final day of the experiment, the preserved sperm was used for fertilization. The ANOVA test showed that the extender and dilution ratio significantly affected the motility and viability of the spermatozoa and egg fertility (P<0.05). The quality decreased after preservation for 144 hours at 4 °C, but after being diluted with Ringer solution at a ratio of 1:40 (sperm:extender, v/v), it produced better results than other extenders at all dilution ratio levels. Therefore, it is recommended that Ringer's solution at a dilution ratio of 1:40 is suitable for preserving walking catfish C. batrachussperm at a refrigerated temperature of 4 °C.

INFLUENCE OF SELECTED PARAMETERS OF MATING VOCALIZATION OF PHEASANTS (PHASIANUS COLCHICUS) ON REPRODUCTIVE SUCCESS

Piotr Czyżowski — 2024-12-23

The purpose of this study was to analyze the mating vocalizations of pheasant roosters (time between successive vocalizations, peak frequency of sound amplitudes) and to demonstrate the relationship between vocalization parameters and the number of hens accumulated by the roosters (reproductive success). The research was conducted based on recordings of the sounds of 6 rolling roosters. A total of 79 isolated vocalizations constituted the study material. The recordings were made using a Zoom H1 portable sound recording device based on two microphones in an X/Y arrangement, enabling correct recording of stereophony. Roosters leading two or one hen were characterized by more active vocalizations, which increases their attractiveness to hens choosing them. Cooks leading two or one hen were characterized by a lower peak frequency of the vocalization sound produced than roosters without hens. This may indicate that roosters making sounds at lower frequencies are more attractive to hens.

ROSE GERANIUM ESSENTIAL OIL RESTORATIVE POTENTIAL ON LEAD ACETATE INDUCED OVARY TOXICITY USING A MOUSE MODEL

Massinissa Madouche — 2024-12-23

It is well known that lead cause tissue damage, dysfunction of folliculogenesis and elevated follicular. Rose geranium essential oil has anti-inflammatory and antioxidant properties and could have therapeutic potential. 18 adult BALB/c mice were used in this study and divided into 3 groups. Group I received physiological water, groups II and III received a single low dose of lead acetate and after 7 days, group III received daily dose of 300µl/Kg of essential oil. Histological samples from group (II) treated with 8mg/Kg of lead acetate reveals altered ovarian structure and those of group (III) treated with 300µl/Kg of geranium essential oil showed an improved ovarian structure. Antral follicles atresia is higher in group (II) than in the other two groups. Our results indicate that a treatment with daily doses of rose geranium essential oil improved the lead-altered ovarian structure and reduced the degeneration of antral follicles.