The Cellulase production by immobilized cells of Candida tropicalis isolated from grasshopper Zonocerus variegatus in Saw dust and rice husk medium
Production of cellulase enzyme by immobilized yeast cell
Blessing Adelabu
a:1:{s:5:"en_US";s:41:"Chrisland University Abeokuta, Ogun State";}Abstract
Immobilization of yeast on local matrix is an approach to reduce immobilization cost. Zonocerus variegatus are known to have specialized gut system that are particularly known for cellulose fermentation. Production of cellulase enzyme from saw dust and rice husk by yeast immobilized on Irvingia gabonensis was investigated. Yeasts were isolated from grasshopper and were screened for cellulase production using Carboxyl Methyl Cellulose agar. Effect of bead size, bead number, inoculum load and bead reusability were investigated. Cellulase production was optimum at 72 hr, 6 numbers of bead, 4 mm bead size, 6 % gel concentration and 4 % inoculum size. There was no obvious loss of activity with re-use of immobilized Candida. tropicalis. This study shows that C. tropicalis isolated from Zonocerus variegatus can be immobilized on I. gabonensis and produce cellululase enzyme from agricultural waste.
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a:1:{s:5:"en_US";s:41:"Chrisland University Abeokuta, Ogun State";}
