EVALUATION OF ECO-EXTRACTION METHODS OF ANTIOXIDANTS AND THEIR ACTIVITIES FROM RETAMA RAETAM TWIGS

Oussama zaoui

a:1:{s:5:"en_US";s:148:"Biogeochemistry of Desert Environments Laboratory, Mathematics and Matter Sciences Faculty, University Kasdi Merbah-Ouargla. Ouargla-30000, Algeria.";}

karima Oughlissi-Dehak

Biogeochemistry of Desert Environments Laboratory, Mathematics and Matter Sciences Faculty, University Kasdi Merbah-Ouargla. Ouargla-30000, Algeria.

Mebarka Bouziane

Biogeochemistry of Desert Environments Laboratory, Mathematics and Matter Sciences Faculty, University Kasdi Merbah-Ouargla. Ouargla-30000, Algeria.

farouk boudou

Research Laboratory of Environment and Health (RLEH), Faculty of Medicine, Sidi-Bel-Abbes, Algeria.

fatiha zaoui

Laboratory of Separation and Purification Technologies, Chemistry Department, Faculty of Sciences, Tlemcen University, BP119 Tlemcen, Algeria.

Amina Benras

Biogeochemistry of Desert Environments Laboratory, Mathematics and Matter Sciences Faculty, University Kasdi Merbah-Ouargla. Ouargla-30000, Algeria.

Mahfoud Hadj-Mahammed

Biogeochemistry of Desert Environments Laboratory, Mathematics and Matter Sciences Faculty, University Kasdi Merbah-Ouargla. Ouargla-30000, Algeria.

abd elghani sehmi

Department of Chemistry, Faculty of Sciences, University of Saïda, Algeria.

Abstract

The purpose of this study was to compare the effectiveness of five different conventional (maceration, reflux, and soxhlet) and non-conventional (microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE)) techniques of extracting secondary metabolites (total phenols, total flavonoid and total condensed tannin Contents) and their antioxidant activity (2,2-diphényl 1-picrylhydrazyle free radical-scavenging ability, and ferric reducing antioxidant power assay) obtained from Retama Raetam twigs using two green solvents (water and ethanol 70%). The aqueous and hydro-ethanolic extracts obtained by MAE revealed the highest contents of total phenols (168.24 ± 0.79 and 182.6 ± 1.29 mg EAG / g DR, respectively), and flavonoids (12.,28 ± 0.92 and 39.97 ± 1.11 mg EC/ g DR, respectively), and exhibited a significant capacity to scavenge free radicals with median inhibitory concentrations (IC₅₀) of 0.45± 0.75 and 0.34 ± 0.39 mg/mL, and a significant iron reducing power with median effective concentrations (EC₅₀) of 0.358 ± 0.2 and 0.28 ± 0.1 mg/mL for the aqueous and hydro-ethanolic extracts, respectively. The MAE was proven to be the most efficient extraction technique for the extraction of antioxidants from Retama Raetam twigs.