Therapeutic Potency of Lemon-Scented Gum (Eucalyptus citriodora) Leaf Extract for the Development of Antibacterial Drug

Joel Uyi Ewansiha

a:1:{s:5:"en_US";s:73:"Department of Microbiology, Modibbo Adama University Yola, Adamawa State.";}

A. J. Adeleke

Modibbo Adama University

Department of Microbiology

Senior Lecturer

 



S. L. Orji

2Bio-resources Development Centre, National Biotechnology Development Agency, Lugbe Abuja, Nigeria.

Department of food sciences

  1. Research Officer


I. D. Kolawole

3Department of Food Science and Technology, Universidade de Sao Paulo, Brazil.

Department of food sciences

Research student. (PhD)


https://orcid.org/0000-0002-2025-7319


Abstract

Objectives: The antibacterial activity, phytochemical components and safe dose of the leaf part of Eucalyptus citriodora was determined using standard methods.
Results: Ten phytochemicals (flavonoid, phenols, alkaloids, tannins, steroids, cardiac glycosides, saponins, terpenes, anthraquinones and resins) were present in other solvent extract but tannins and resinswere absent in the aqueous extract. All test organisms were susceptible to ethyl acetate crude extract with mean inhibition zone diameter (IZD) range from 14.00±0.00 to 21.66±0.88 mm but were not susceptible to the aqueous extract; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values from 7.5 to 60 mg/ml respectively were obtained. Eight fractions were obtained from column chromatography of which 5 were active; however, 3 shows significant (p<0.05) activity with IZD of 26.00±0.00 mm, 23.6±0.33 mm and 20.33±0.33 mm respectively while their corresponding MIC/MBC were 3.75, 1.88 and 3.25 mg/ml/60, 30 and 30 mg/ml. In the same vein, 28, 18 and 17 compounds were detected by gas chromatography in the 3 significantly active fractions. Generally, the alkane group of compounds was the most detected, but for emphasis, Tetracosane (14.03% of ECE1), Octadecanal (59.54% of ECE4) and Decane (13.70% of ECE5) were the most abundant in the three active fraction. The LD50 for the extract was 707.10 mg/kg bw while mild tissue necrosis and degenerative glomeruli were observed in the liver and kidney pathology of experimental rats administered with 1000 mg/ml of extract.
Conclusion: Therefore, Eucalyptus citriodora leaf extract indicated antibacterial potency and therefore considered for drug development.


Keywords:

Eucalyptus citriodora, ethyl acetate and aqueous fractions, acute toxicity, antibacterial, alkane, compounds


Aboshora W., Lianfu Z., Dahir M., Qingran M., Qingrui S., Jing L., Al-Haj Nqm, Amma r A.   Google Scholar

Effect of extraction method and solvent power on polyphenol and flavonoid levels in Hyphaene   Google Scholar

thebaica L. Mart Arecaceaedoum fruit, and its antioxidant and antibacterial activities.   Google Scholar

Tropical Journal of Pharmaceutical Research,13: 2057–2063.   Google Scholar

Acevedo R.P., Strongm. T. 2012. Catalogue of the seed plants of the West Indies. Smithsonian   Google Scholar

Contributions to Botany, 98: 1192.   Google Scholar

Acuna G.J. 2015. Undesirable plants in Cuban crops. Academia de Ciencias de Cuba, Havana,   Google Scholar

pp. 241.   Google Scholar

Altemimi A.W., Watson D.G., Kinsel M., Lightfoot D.A. 2015. Simultaneous extraction, optimization,   Google Scholar

and analysis of flavonoids and polyphenols from peach and pumpkin extracts using   Google Scholar

a tlc-densitometric method. Chemistry Central Journal, 9: 1–15.   Google Scholar

Altemimi A., Lakhssassi N., Baharlouei A., Watson D. 2017. Phytochemicals: Extraction, isolation,   Google Scholar

and identification of bioactive compounds from plant extracts. Plants, 6: 42, doi:   Google Scholar

3390/plants6040042.   Google Scholar

Arag e M., Eguale T., Giday M. 2022. Evaluation of antibacterial activity and acute toxicity of   Google Scholar

methanol extracts ofArtemisia absinthium, Datura stramonium, and Solanum anguivi. Infection   Google Scholar

and Drug Resistance, 15: 1267–1276.   Google Scholar

Baynesag ne S., Berhane N., Sendeku W., Ai L. 2017. Antibacterial activity of Datura stramonium   Google Scholar

against standard and clinical isolate pathogenic microorganisms. Journal Medicinal   Google Scholar

Plants Research, 11(3): 501–506.   Google Scholar

Belayneh Y.M., Birhanu Z., Birru E.M, Getenet G. 2019. Evaluation of in vivo antidiabetic,   Google Scholar

antidyslipidemic, and in vitro antioxidant activities of hydromethanolic root extract of Datura   Google Scholar

stramonium L. (Solanaceae). Journal of Experimental Pharmacology, 11: 29–38.   Google Scholar

Zhao B., Jiang X., Cao J., Huang K. 2019. Biostatistical analysis on medicinal plant toxicity.   Google Scholar

Biomedical Journal of Scientific and Technical Research, 18(5): 13890–13896, doi: 10.26717/   Google Scholar

BJSTR.2019.18.003212.   Google Scholar

Brinda C., Mohan K.R. 2016. Evaluation of antioxidant, antibacterial activity of ethanolic extract   Google Scholar

in the leaves of Combretum Albidum and gas chromatography-mass spectrometry analysis.   Google Scholar

Asian Journal of Pharmaceutical and Clinical Research, 9(4): 325–329.   Google Scholar

Clinica l and Lab oratory Standard Institute (CLSI). 2012. Performance standards for antimicrobial   Google Scholar

susceptibility testing; seventeenth information supplement, 27(1): 100–117.   Google Scholar

Ewansiha J.U., Garba S.A., Galadima M., Daniyan S.Y., Busari M.B., Doughari J.H. 2017.   Google Scholar

Bioactivity of chromatographic fractions from eucalyptus citriodora leaf against some bacterial   Google Scholar

pathogens. International Journal of Clinical Medicine Research, 4(1): 1–14.   Google Scholar

Ewansiha J.U., Tilon G., Kolawole I.D., Orji S.L. 2020. Inhibitory potential of coconut and   Google Scholar

carrot oil extracts on some bacterial skin microflora.African Journal of Biology and Medical   Google Scholar

Research, 3(3): 15–21.   Google Scholar

Ewansiha J.U. 2020. Evaluation of antibacterial potency of Citrus Limon (Lemon) juice against   Google Scholar

some pathogenic organisms as alternative source of chemotherapy.European Journal of Biology   Google Scholar

and Biotechnology, doi: 10.24018/2020.1.1.12.   Google Scholar

Ewansiha J.U., Garba S.A., Galadima M., Daniyan S.Y, Busari M.B., Doughari J.H. 2017.   Google Scholar

Bioactivity of chromatographic fractions from Eucalyptus citriodora leaf against some bacterial   Google Scholar

pathogens. International Journal of Clinical Medicine Research, 4(1): 1–14.   Google Scholar

Ewansiha J.U., Garba S.A., Mawak J.D., Oyewole O.A. 2012. Antimicrobial activity of Cymbopogon   Google Scholar

citratus (Lemon Grass) and its phytochemical properties. Frontiers in Science,   Google Scholar

(6): 214–220.   Google Scholar

Geleta B., Eyasu M., Kebam o A., Mekonnen E., Abebe A. 2015. In vitro vasodilatory effect of   Google Scholar

aqueous leaf extract of Thymus serrulatuson thoracic aorta of Guinea pigs. Asian Pacific Journal   Google Scholar

of Tropical Biomedicine, 5(1): 15–18.   Google Scholar

Getaneh S., Girma Z. 2014. Ethnobotanical study of medicinal plants in Debre Libanosereda,   Google Scholar

central Ethiopia. African Journal of Plant Science, 8(7): 366–379,doi:10.5897/AJPS2013.1041.   Google Scholar

Ghadir A.E, Abeer F.A, Eman S.R. 2014. Evaluation of the antioxidant and antibacterial properties   Google Scholar

of various solvents extracts of A. squamosal L. leaves. Arabian Journal of Chemistry,   Google Scholar

: 227–233.   Google Scholar

Griffith O.W, Ginter P.W. 2017. The placenta as a model for understanding the origin and evolution   Google Scholar

of vertebrate organs. Nature Ecology & Evolution, 1(4): 72.   Google Scholar

Haj ir B.A., Alaa I.M., Khansa A.A, Naga I., Wdeea A.A., Monier N.H. (2016). Evolution of antimicrobial,   Google Scholar

antioxidant potentials and phytochemical studies of three solvent extracts of five   Google Scholar

species from Acacia used in sudanese ethnomedicine. Advances in Microbiology, 6: 691–698.   Google Scholar

Haj ir B.A., Alaa I.M, Khansa A.A. 2016. Evolution of antimicrobial, antioxidant potentials and   Google Scholar

phytochemical studies of three solvent extracts of five species from acacia used in sudanese   Google Scholar

ethnomedicine. Advances in Microbiology, 6: 691–698.   Google Scholar

Innalegwu D.A., Ewansiha J.U., Amuda O.A., Vivian N. 2016. Phytochemical and anti-microbial   Google Scholar

study of seventy percent methanol leaf extract of Terminalia microptera against selected   Google Scholar

pathogenic bacteria. Journal of Advances in Medical and Pharmaceutical Sciences,   Google Scholar

(1): 1–8.   Google Scholar

Ewansiha J.U., Ugo C.E., Kolawole D.I, Orji L.S. 2021. Antibacterial activities of Hyphaene   Google Scholar

thebaica (Doum Palm) fruit extracts against intestinal microflora and potential constipation   Google Scholar

associated pathogens in Yola Metropolis, Nigeria,Tanzania Journal of Science, 47(1): 104–111.   Google Scholar

La M.P, Li C., Li L. 2012. New bioactive sulfated alkenes from the sea cucumber Apostichopus japonicus.   Google Scholar

Chemistry & Biodiversity, 9(6): 1166–1171, doi: 10.1002/cbdv.201100324.   Google Scholar

Malik M., Li L., Zhao X. 2014. Lethal synergy involving bicyclomycin: an approach for reviving   Google Scholar

old antibiotics. Journal of Antimicrobial Chemotherapy, 9(12), 3227–3235.   Google Scholar

Mammen D., Bapat S., Sane R. 2012. An investigation to variation in constituents in the fruits of   Google Scholar

Terminalia chebula Retz at different maturity stages. International Journal of Pharmacy and   Google Scholar

Biological Sciences, 3(1): 416–419.   Google Scholar

Mohanapriya M., Lalitha R., Raj endran R. 2013. Health and medicinal properties of lemon   Google Scholar

(Citrus limonum). International Journal of Ayurvedic and Herbal Medicine, 3(1), 1095–1100.   Google Scholar

Odeleye A.A, Obameso J.O. 2022. Activities ofDatura stramonium extracts against Clinical   Google Scholar

Pathogens. South Asian Journal of Research in Microbiology, 13(2): 12–18, doi: 10.9734/   Google Scholar

SAJRM/2022/v13i230292.   Google Scholar

Okokon J.E, Simeon J.O, Umoh E.E. 2018. Hepatoprotective activity of extract of Homalium Letestui   Google Scholar

stem against carbon tetrachloride-induced liver injury. Avicenna Journal of Phytomedicine,   Google Scholar

(4): 1–11.   Google Scholar

Okokon J.E, Simeon J.O., Umoh E.E. 2018. Hepatoprotective activity of extract of Homalium   Google Scholar

Letestui stem against carbon tetrachloride-induced liver injury. Avicenna Journal Phytomed,   Google Scholar

(4): 1–11.   Google Scholar

Pradhan P., Tama ng J.P. 2019. Phenotypic and genotypic identification of bacteria isolated from   Google Scholar

traditionally prepared dry starters of the Eastern Himalayas. Frontiers in Microbiology,   Google Scholar

: 1–15, doi: 10.3389/fmicb.2019.02526.   Google Scholar

Rehma n S., Nadeem U., Mehwish J., Riffa t Z., Hina A. 2022. Light and scanning electron microscopy   Google Scholar

of Datura stramonium L. extract and its biological applications. Microscopy Research   Google Scholar

and Technique, 85(8): 2997–3004, doi: 10./jemt.24148.   Google Scholar

Sardar A.F., Muja ddadur-Rehman K., Naumank., Khan S.A. 2012. Antimicrobial activity   Google Scholar

of Eucalyptus Tereticornisand comparison with daily life antibiotics.International Journal of   Google Scholar

Pharmaceutical Sciences Review and Research, 12(1): 21–29.   Google Scholar

Sarswati N.O., Kuldeep S.N., Lalit M.T., Puran S.M., Jai C.R. Veena P., Sandeep S., Amit P.   Google Scholar

Variation in essential oil composition and anti-microbial activity of Indian Oregano   Google Scholar

(Origanum vulgare L.) population from Indian Himalayan Region (IHR). Journal of Medicinal   Google Scholar

Plants Research, 7(46): 3375–3384.   Google Scholar

Sarswati, N.O., Kuldeep, S.N. and Lalit S.N. 2013. Variation in essential oil composition and   Google Scholar

antimicrobial activity of Indian Oregano (Origanum vulgare L.) population from Indian Himalayan   Google Scholar

Region (IHR). Journal of Medicinal Plants Research, 7(46), 3375–3384.   Google Scholar

Tojola O.B, Laj ide L. Owolab i B.J, Olaleye M.T., Okoh S.O. 2019. Phytochemical screening   Google Scholar

& antibacterial activity of ethyl acetate & methanol extracts of Annona muricata aerial part.   Google Scholar

Journal of Medicinal Plants Studies, 7(6): 1–5.   Google Scholar

Waza S.A., Anthony P., Dar S. 2015. Phytochemical analysis, antioxidant and antimicrobial   Google Scholar

activities of methanolic extract of Datura stramonium seeds. International Journal   Google Scholar

of Pharmaceutical Sciences and Research, 6: 3021–3026.   Google Scholar

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Published
2023-06-22

Cited by

Ewansiha, J. U., Adeleke , J. A., Orji, . S. L., & Kolawole, I. D. (2023). Therapeutic Potency of Lemon-Scented Gum (Eucalyptus citriodora) Leaf Extract for the Development of Antibacterial Drug. Polish Journal of Natural Sciences, 37(3). https://doi.org/10.31648/pjns.8143

Joel Uyi Ewansiha 
a:1:{s:5:"en_US";s:73:"Department of Microbiology, Modibbo Adama University Yola, Adamawa State.";}
A. J. Adeleke 
Modibbo Adama University
<p>Department of Microbiology</p> <p>Senior Lecturer</p> <p>&nbsp;</p>  Nigeria

Department of Microbiology

Senior Lecturer

 


S. L. Orji 
2Bio-resources Development Centre, National Biotechnology Development Agency, Lugbe Abuja, Nigeria.
<p>Department of food sciences</p> <ol> <li class="show">Research Officer</li> </ol>  Nigeria

Department of food sciences

  1. Research Officer

I. D. Kolawole 
3Department of Food Science and Technology, Universidade de Sao Paulo, Brazil.
<p>Department of food sciences</p> <p>Research student. (PhD)</p>  Brazil
https://orcid.org/0000-0002-2025-7319

Department of food sciences

Research student. (PhD)









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